Cell Permeant Polyphosphoinositide-binding Peptides That Block Cell Motility and Actin Assembly

Actin-Based Cell Motility and Cell Locomotion

they move. Deadhesion/tail retraction may limit moveIntroduction ment rate in such cells. In weakly adhesive, fast moving Most animal cell types possess the capacity to move cells such as amoebae and white blood cells, the tail is over or through a substrate, and cell locomotion plays more rounded and this step is more efficient. Adhesion a key role in bothnormal physiology and disease.Certain ...

1 Control of Actin Assembly Dynamics in Cell Motility

Motile processes at the origin of cell migration, cell division, synaptic plasticity and endocytosis, but also morphogenetic processes that define anterior-posterior and ventral-dorsal polarity of the embryo, or left-right asymmetry, are driven by spatially and temporally controlled assembly of actin filaments (1-4). Cell biological studies and reconstituted motility assays indicate that polari...

Control of actin assembly dynamics in cell motility.

Therapeutic effects of cell-permeant peptides that activate G proteins downstream of growth factors.

In eukaryotes, receptor tyrosine kinases (RTKs) and trimeric G proteins are two major signaling hubs. Signal transduction via trimeric G proteins has long been believed to be triggered exclusively by G protein-coupled receptors (GPCRs). This paradigm has recently been challenged by several studies on a multimodular signal transducer, Gα-Interacting Vesicle associated protein (GIV/Girdin). We re...

Arginylation of beta-actin regulates actin cytoskeleton and cell motility.

Posttranslational arginylation is critical for mouse embryogenesis, cardiovascular development, and angiogenesis, but its molecular effects and the identity of proteins arginylated in vivo are unknown. We found that beta-actin was arginylated in vivo to regulate actin filament properties, beta-actin localization, and lamella formation in motile cells. Arginylation of beta-actin apparently repre...